Biological Safety Cabinet Class III

Biological Safety Cabinet Class III

Laboratory isolator suitable for BSL 3, for personnel and product protection
Weighing - Containment Isolator

A laboratory isolator suitable for BSL 3 provides a high degree of protection for personnel working with most hazardous materials. Additionally, due to its “A” class cleanliness and laminar airflow product protection is also ensured. This isolator can be used in pharmacies or hospitals for preparation (dilution and dosing) of cytostatics as well as in laboratories for work with any dangerous and biological materials.


  • Working chamber and a pair of material airlocks
  • Options for two or four gloves in the working chamber
  • Leak tightness class 3 according to ISO 10648-2
  • “A” Class cleanliness according to EU GMP standard
  • Option to decontaminate with H2O2
  • Electronic control system for automatic adjustment of basic operating modes managed by a Siemens PLC
  • HMI Colour touchscreen
  • Progress visualisation of each of the production steps and current parameters
  • Jacket material: S235JR steel grade, powder coated surface
  • Chamber interior material: AISI 304 stainless steel, powder coated surface
  • Work surface material: AISI 316L stainless steel, polished
  • Inlet filtration HEPA H14 - “A” Class cleanliness
  • Safe filtration of exhaust air with double HEPA H14 filters
  • Easy-to-clean interior and exterior surfaces
  • Sliding trays between Airlocks and the Main chamber for easy material transfer
  • Oval flanges with gloves for improved material handling
  • Compact dimensions – easy relocation through a doorway in one piece
  • Laminar flow 0,45 m/s ±20%
  • Negative pressure mode

Laminar Flow

Laminar flow is used for “A” class cleanliness, when unidirectional airflow must be ensured in a vertical orientation. A laminar frame is used to direct the flow— it is comprised of a special very finely perforated fabric. The velocity of laminar flow produced by the isolator technology amounts to 0,45 m/s ±20%. HEPA filters (H14), with an efficiency of 99.995% are used to filter incoming and outgoing air. This system is often coupled with air re-circulation inside the chamber.

Biological Safety Cabinet Class III - Laminar flow

Negative Pressure

An HVAC system allows for the formation of negative pressure inside the isolator. This function is mostly used in case of isolation failure - compromised leak-proofness - and assists in protecting personnel.

Another level of protection for critical points in the process is the creation of a pressure cascade, so that the vicinity where the dangerous substance is handled is in the location with the highest negative pressure. This minimizes the risk for contamination of other chambers or the surroundings.

In order to prevent damage to the equipment caused by insufficient pressure a pneumatic valve is used for device protection. This protective function prevents the pressure from dropping even further thus saving the technology from damage.

Biological Safety Cabinet Class III - Negative pressure


  1. Main chamber
  2. Airlock 1
  3. Airlock 2
  4. Main switch
  5. HMI panel
  6. Visual and acoustic alarm
  7. Front window
  8. Flange and gloves
  9. Hinge with position control
  10. Switchboard
  11. Service panel
  12. Inlet



Biological Safety Cabinet Class lll - Equipment

Allows material transport to the working chamber (Airlock 1) and from the working chamber (Airlock 2).

Double door protection

Biological Safety Cabinet Class lll - Equipment

During material transport only one of the pass-through chamber doors can remain open, thereby preventing connection of the working chamber with the surroundings.

Visual and acoustic alarm

Biological Safety Cabinet Class lll - Equipment

A beacon light and an acoustic alarm provide a quick audible and visual assessment of operation and failure states for the user.

Front window

Biological Safety Cabinet Class lll - Equipment

A gas spring assists with easy opening. Hinged with position control for safe closure. Installed with safety glass and fitted with flanges for sleeves. Sensors monitor safe closure of the window.

Hinge with position control

Biological Safety Cabinet Class lll - Equipment

Hinges with built-in position sensors. The control system monitors the current status of the front window and produces an alarm in case two doors are opened simultaneously.


Biological Safety Cabinet Class lll - Equipment

Switchboard with protection IP44.

Rear built-in panel

Biological Safety Cabinet Class lll - Equipment

This interchangeable rear panel allows accessories to be built in according to customer requirement. With the option to utilise a standardised design of the rear panel or a specific tailor-made design.

Laminar frame

Biological Safety Cabinet Class lll - Equipment

A laminar frame is used for orientation of airflow that contains an extra fine perforated steel sheet and special fabric.

230 V electric sockets, IP66 rated protection

Biological Safety Cabinet Class lll - Equipment

In the interior rear wall of the main chamber SCAME sockets are placed and covered by a mechanically lockable safety latch. Our standard delivery consists of two IP66 sockets in the main chamber.

Mechanical door-locks

Biological Safety Cabinet Class lll - Equipment

The entrance door of the pass-through cabin is equipped with a mechanical door-locking mechanism and hinges with positional control.

In-chamber Sensors

Biological Safety Cabinet Class lll - Equipment

Set of sensors located inside the main chamber. Controlling sensor for air velocity is placed 250 mm above the air inlet. For monitoring of temperature and relative humidity iss used second sensor.


Biological Safety Cabinet Class lll - Equipment

The working surface is made from highly polished AISI 316 stainless steel. For optimum airflow, air is exhausted through a perforation on the sides of the working surface.


  • Sliding trays between the Airlock and the Main chamber
    • The airlocks are equipped with a sliding tray for improved ergonomics and easy transfer of material.
  • Built-in LED lights
    • Built-in LED lights ensure an illumination level of at least 500 lux.
  • Rounded edges/corner inside the working chamber
    • Rounded corners and highly polished surfaces allow easy cleaning of the chamber.
    • The chambers are precision-welded which guarantees their tightness.

Safety Features

  • Safety glass
    • Tempered safety glass is used for the front windows and ports. This glass has improved mechanical properties and is durable. When damaged it forms small chunks which reduce the risk of injury from cuts.
  • Safe replacement of sleeves
    • Our glove/sleeve flanges have two grooves. The first is for sealing the glove and the second is for an o-ring. This design allows a standardised procedure for the safe replacement of gloves.
  • Safe main filter replacement
  • Automatic leak tightness test


  • Weighing scale
    • Optional integration of any type of weighing scale and terminal.
  • Bar-code reader
    • Integration of a bar-code reader.
  • LCD screen
    • The rear wall of the working chamber can be equipped with an LCD screen, while applications for the dilution of cytostatics can be accessed on a built-in PC.
  • H2O2 decontamination
    • Sterilization can be performed using vaporised hydrogen peroxide. These vapours are generated by an additional external device – Puriter (our hydrogen peroxide vapour generator).
  • Additional equipment and media connection
    • Interchangeable rear wall and options to integrate additional equipment (incubators, an LED screen, balance and weighing terminal, etc.) or include a media connection.
  • Accessories
    • The rear panel can be fitted with wire trays, hangers and other accessories made of AISI 304 stainless steel.
  • Independent UPS power supply
    • An independent UPS (Uninterruptible Power Supply) can be installed inside the isolator. In the event of an interruption in the supply of electricity, short-term operation is maintained through a power supply in the isolator so that the process and the environment may be secured.
  • Glove holder
    • Holders for gloves are utilised in isolators where sterilisation with vaporised hydrogen peroxide is used.


HMI Panel

This isolator is equipped with a Siemens Simatic electronic control system. The system is used to control, manage and regulate all operating and emergency conditions. System control is via a colour touch panel which features all control buttons and displays, process data and fault messages. The control panel is situated in the upper structure above the chambers.

Overview of Functions

  • User login and automatic log-off countdown timer
  • Socket control icons
  • Illumination control icons
  • Chamber interior physical values measurement display
  • Device operation modes menu, Alarms and Settings menu

The control system can transmit all operating data from the isolator to a master system where the data is archived.

The control system is also used for graphically displaying archived data and trend evaluation. This data can be transmitted to a personal computer or master system for detailed data processing.


Biological Safety Cabinet Class lll

Dimensions in mm - Biological safety cabinet class lll

Width 2440
Height 1950
Front height 1450
Depth 770
Main chamber working plate height 825
Glove position 1090
Main chamber usable depth space 596

Dimensions in mm - Main chamber

Width 1340
Depth 600
Height 695
Main chamber total volume 0,55 m3

Dimensions in mm - Airlock 1, Airlock 2

Width 550
Depth 460
Height 665
Airlock Total volume 0,17 m3


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